Engineering a chimeric pyrroloquinoline quinone glucose dehydrogenase: improvement of EDTA tolerance, thermal stability and substrate specificity.

@article{Yoshida1999EngineeringAC,
  title={Engineering a chimeric pyrroloquinoline quinone glucose dehydrogenase: improvement of EDTA tolerance, thermal stability and substrate specificity.},
  author={Hironori Yoshida and Kazutaka Kojima and Arief Budi Witarto and Koji Sode},
  journal={Protein engineering},
  year={1999},
  volume={12 1},
  pages={63-70}
}
An engineered Escherichia coli PQQ glucose dehydrogenase (PQQGDH) with improved enzymatic characteristics was constructed by substituting and combining the gene-encoding protein regions responsible for EDTA tolerance, thermal stability and substrate specificity. The protein region responsible for complete EDTA tolerance in Acinetobacter calcoaceticus, which is recognized as the indicator of high stability in co-factor binding, was elucidated. The region is located between 32 and 59% from the N… CONTINUE READING
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Acta., 218,137–142

  • Anal. Chim
  • Received August
  • 1998

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