Engineering Pseudomonas putida S12 for efficient utilization of D-xylose and L-arabinose.

@article{Meijnen2008EngineeringPP,
  title={Engineering Pseudomonas putida S12 for efficient utilization of D-xylose and L-arabinose.},
  author={Jean-Paul Meijnen and Johannes H. De Winde and Harald J. Ruijssenaars},
  journal={Applied and environmental microbiology},
  year={2008},
  volume={74 16},
  pages={5031-7}
}
The solvent-tolerant bacterium Pseudomonas putida S12 was engineered to utilize xylose as a substrate by expressing xylose isomerase (XylA) and xylulokinase (XylB) from Escherichia coli. The initial yield on xylose was low (9% [g CDW g substrate(-1)], where CDW is cell dry weight), and the growth rate was poor (0.01 h(-1)). The main cause of the low yield was the oxidation of xylose into the dead-end product xylonate by endogenous glucose dehydrogenase (Gcd). Subjecting the XylAB-expressing P… CONTINUE READING

From This Paper

Figures, tables, and topics from this paper.

Citations

Publications citing this paper.
Showing 1-10 of 29 extracted citations

Genetic tools for reliable gene expression and recombineering in Pseudomonas putida

Journal of Industrial Microbiology & Biotechnology • 2017
View 2 Excerpts

References

Publications referenced by this paper.
Showing 1-10 of 31 references

Similar Papers

Loading similar papers…