Engineering NADH/NAD+ ratio in Halomonas bluephagenesis for enhanced production of polyhydroxyalkanoates (PHA).

@article{Ling2018EngineeringNR,
  title={Engineering NADH/NAD+ ratio in Halomonas bluephagenesis for enhanced production of polyhydroxyalkanoates (PHA).},
  author={Chen Ling and Guan-Qing Qiao and Bo-Wen Shuai and Karel Olavarria and Jin Yin and Ruijuan Xiang and Kun-Nan Song and Yun-Hao Shen and Yingying Guo and Guo-qiang Chen},
  journal={Metabolic engineering},
  year={2018},
  volume={49},
  pages={
          275-286
        }
}

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References

SHOWING 1-10 OF 87 REFERENCES
Effect of nitrogen availability on the poly-3-d-hydroxybutyrate accumulation by engineered Saccharomyces cerevisiae
TLDR
In contrast to what was observed in natural PHB-producing bacteria, nitrogen deficiency did not improve PHB accumulation in S. cerevisiae, and the excess available carbon from xylose was shunted into glycogen, indicating a significant gluconeogenic activity onxylose.
Production of medium chain length polyhydroxyalkanoate in metabolic flux optimized Pseudomonas putida
TLDR
Overproduction of pyruvate dehydrogenase subunit AcoA in the P. putida KT2440 wild type and the Δgcd mutant strains led to an increase of PHA production, and NADPH seems to be quantitatively consumed for efficient PHA synthesis, since a direct relationship between low levels of NADPH and high concentrations of the biopolymer were observed.
Engineering Halomonas TD01 for the low-cost production of polyhydroxyalkanoates.
Regulation of the tricarboxylic acid cycle and poly-beta-hydroxybutyrate metabolism in Azotobacter beijerinckii grown under nitrogen or oxygen limitation.
TLDR
Respiratory activity, as measured by the QO2 value, increased with oxygen supply rate, and changes are discussed in relation to respiratory protection of nitrogenase and poly-beta-hydroxybutyrate metabolism in A. beijerinckii.
Anaerobic poly-3-d-hydroxybutyrate production from xylose in recombinant Saccharomyces cerevisiae using a NADH-dependent acetoacetyl-CoA reductase
TLDR
This study reports a novel approach for boosting PHB accumulation in S. cerevisiae by replacement of the commonly used AAR from C. necator with the NADH-dependent alternative from A. vinosum, and to the best of the authors' knowledge, it is the first demonstration of anaerobic PHB synthesis from xylose.
Directed Evolution and Structural Analysis of NADPH-Dependent Acetoacetyl Coenzyme A (Acetoacetyl-CoA) Reductase from Ralstonia eutropha Reveals Two Mutations Responsible for Enhanced Kinetics
TLDR
Comparative three-dimensional structural analysis of wild-type and highly active PhaB mutants revealed that the beneficial mutations affected the flexibility around the active site, which in turn played an important role in substrate recognition.
The Soluble and Membrane-bound Transhydrogenases UdhA and PntAB Have Divergent Functions in NADPH Metabolism of Escherichia coli*
Pentose phosphate pathway and isocitrate dehydrogenase are generally considered to be the major sources of the anabolic reductant NADPH. As one of very few microbes, Escherichia coli contains two
...
...