Engineering Herbicide Metabolism in Tobacco and Arabidopsis with CYP76B1, a Cytochrome P450 Enzyme from Jerusalem Artichoke1

  title={Engineering Herbicide Metabolism in Tobacco and Arabidopsis with CYP76B1, a Cytochrome P450 Enzyme from Jerusalem Artichoke1},
  author={L. Didierjean and L. Gondet and R. Perkins and Sze-Mei Cindy Lau and H. Schaller and D. P. O'keefe and D. Werck-Reichhart},
  journal={Plant Physiology},
  pages={179 - 189}
  • L. Didierjean, L. Gondet, +4 authors D. Werck-Reichhart
  • Published 2002
  • Medicine, Biology
  • Plant Physiology
  • The Jerusalem artichoke (Helianthus tuberosus) xenobiotic inducible cytochrome P450, CYP76B1, catalyzes rapid oxidative dealkylation of various phenylurea herbicides to yield nonphytotoxic metabolites. We have found that increased herbicide metabolism and tolerance can be achieved by ectopic constitutive expression of CYP76B1 in tobacco (Nicotiana tabacum) and Arabidopsis. Transformation with CYP76B1 conferred on tobacco and Arabidopsis a 20-fold increase in tolerance to linuron, a compound… CONTINUE READING
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