Engineering Escherichia coli for propionic acid production through the Wood–Werkman cycle

  title={Engineering Escherichia coli for propionic acid production through the Wood–Werkman cycle},
  author={R A Gonzalez-Garcia and Tim McCubbin and Mark S. Turner and Lars Keld Nielsen and Esteban Marcellin},
  journal={Biotechnology and Bioengineering},
  pages={167 - 183}
Native to propionibacteria, the Wood–Werkman cycle enables propionate production via succinate decarboxylation. Current limitations in engineering propionibacteria strains have redirected attention toward the heterologous production in model organisms. Here, we report the functional expression of the Wood–Werkman cycle in Escherichia coli to enable propionate and 1‐propanol production. The initial proof‐of‐concept attempt showed that the cycle can be used for production. However, production… Expand
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