Engineering Escherichia coli for propionic acid production through the Wood–Werkman cycle

@article{GonzalezGarcia2019EngineeringEC,
  title={Engineering Escherichia coli for propionic acid production through the Wood–Werkman cycle},
  author={R A Gonzalez-Garcia and Tim McCubbin and Mark S. Turner and Lars Keld Nielsen and Esteban Marcellin},
  journal={Biotechnology and Bioengineering},
  year={2019},
  volume={117},
  pages={167 - 183}
}
Native to propionibacteria, the Wood–Werkman cycle enables propionate production via succinate decarboxylation. Current limitations in engineering propionibacteria strains have redirected attention toward the heterologous production in model organisms. Here, we report the functional expression of the Wood–Werkman cycle in Escherichia coli to enable propionate and 1‐propanol production. The initial proof‐of‐concept attempt showed that the cycle can be used for production. However, production… Expand
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References

SHOWING 1-10 OF 52 REFERENCES
Construction of a novel anaerobic pathway in Escherichia coli for propionate production
TLDR
This work has built the basis for industrial propionate production with E. coli by combining reduced TCA cycle with the native sleeping beauty mutase (Sbm) cycle to construct a redox balanced and energy viable fermentation pathway for anaerobic propionates production. Expand
Genome-scale model guided design of Propionibacterium for enhanced propionic acid production
TLDR
Together, these strategies demonstrate that in silico design strategies are predictive and can be used to reduce by-product formation in Propionibacterium. Expand
Pathway engineering of Propionibacterium jensenii for improved production of propionic acid
TLDR
Propionic acid production in P. jensenii with ppc overexpression as well as ldh deletion was investigated, which resulted in further increases in PA titer to 34.93 ± 2.99 g·L−1 in a fed-batch culture. Expand
Metabolic engineering of Propionibacterium freudenreichii for n-propanol production
TLDR
This is the first report on producing n-propanol by metabolically engineered propionibacteria, which offers a novel route to produce n- Propanol from renewable feedstock, and possibly a new way to boost propionic acid fermentation. Expand
Manipulating the sleeping beauty mutase operon for the production of 1-propanol in engineered Escherichia coli
TLDR
The study has provided a novel route for 1-propanol production in E. coli, which is subjected to further improvement by identifying limiting conversion steps, shifting major carbon flux to the productive pathway, and optimizing gene expression and culture conditions. Expand
Engineering Escherichia coli with acrylate pathway genes for propionic acid synthesis and its impact on mixed-acid fermentation
TLDR
A novel way of synthesising propionic acid by employing a non-native, user-friendly organism through metabolic engineering is reported, which caused an increased lactate production in E. coli and could be attributed to the low activity of the recombinant enzymes. Expand
Metabolic engineering of Escherichia coli for direct production of 1,4-butanediol.
TLDR
This work engineered the E. coli host to enhance anaerobic operation of the oxidative tricarboxylic acid cycle, thereby generating reducing power to drive the BDO pathway, leading to a strain of Escherichia coli capable of producing 18 g l(-1) of this highly reduced, non-natural chemical. Expand
Investigation on the anaerobic propionate degradation by Escherichia coli K12
TLDR
This is the first study describing the influence of RNase R on the anaerobic metabolism of E. coli and revealing a posttranscriptional regulation of the prpBCDE‐gene cluster encoding the necessary enzymes for propionate metabolism. Expand
Linking genotype and phenotype in an economically viable propionic acid biosynthesis process
TLDR
A strain capable of producing PA exceeding the commercial needs is obtained using genome shuffling and it is suggested that a mutation in the cytochrome C biogenesis gene, coupled with ATP production through the Wood–Werkman cycle, may be responsible for the increased PA production. Expand
Improved Tolerance of Escherichia coli to Propionic Acid by Overexpression of Sigma Factor RpoS
Propionic acid (PA) is an economically important compound, but large-scale microbial production of PA confronts obstacle such as acid stress on microbial cells. Here, we show that overexpressingExpand
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