Engineering E. coli for improved microaerobic pDNA production

  title={Engineering E. coli for improved microaerobic pDNA production},
  author={Karim E. Ja{\'e}n and Daniela Vargas Vel{\'a}zquez and Frank Delvigne and Juan-Carlos Sigala and Alvaro R. Lara},
  journal={Bioprocess and Biosystems Engineering},
Escherichia coli strains W3110 and BL21 were engineered for the production of plasmid DNA (pDNA) under aerobic and transitions to microaerobic conditions. The gene coding for recombinase A (recA) was deleted in both strains. In addition, the Vitreoscilla hemoglobin (VHb) gene (vgb) was chromosomally inserted and constitutively expressed in each E. coli recA mutant and wild type. The recA inactivation increased the supercoiled pDNA fraction (SCF) in both strains, while VHb expression improved… CONTINUE READING

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