Engineering Clostridium acetobutylicum for alcohol production.

  title={Engineering Clostridium acetobutylicum for alcohol production.},
  author={Xiaohu Hou and Wanfeng Peng and Lian Xiong and Chao Huang and Xuefang Chen and Xinde Chen and Weiguo Zhang},
  journal={Journal of biotechnology},
  volume={166 1-2},
While Clostridium acetobutylicum has been used for large-scale butanol production (ABE fermentation), its by-product acetone cannot be used as a biofuel. In this study, C. acetobutylicum was engineered for alcohol titers (butanol plus ethanol). The adc gene was inactivated to eliminate acetone production, and glutathione biosynthetic capability was introduced into C. acetobutylicum to improve the strain's robustness by expressing Escherichia coli's gshAB genes in the adc locus. Acetone… CONTINUE READING
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