Engineering CRISPR/Cpf1 with tRNA promotes genome editing capability in mammalian systems

  title={Engineering CRISPR/Cpf1 with tRNA promotes genome editing capability in mammalian systems},
  author={Han Wu and Qishuai Liu and Hui Shi and Jingke Xie and Quanjun Zhang and Zhen Ouyang and Nan Li and Yi Yang and Zhaoming Liu and Yu Zhao and Chengdan Lai and Degong Ruan and Jiangyun Peng and Weikai Ge and Fangbing Chen and Nana Fan and Qin Jin and Yanhui Liang and Ting Lan and Xiaoyu Yang and Xiao Shaun Wang and Zhiyong Lei and Pieter A. F. M. Doevendans and Joost P G Sluijter and Kepin Wang and Xiaoping Li and Liangxue Lai},
  journal={Cellular and Molecular Life Sciences},
CRISPR/Cpf1 features a number of properties that are distinct from CRISPR/Cas9 and provides an excellent alternative to Cas9 for genome editing. To date, genome engineering by CRISPR/Cpf1 has been reported only in human cells and mouse embryos of mammalian systems and its efficiency is ultimately lower than that of Cas9 proteins from Streptococcus pyogenes. The application of CRISPR/Cpf1 for targeted mutagenesis in other animal models has not been successfully verified. In this study, we… CONTINUE READING
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