Engineering Bispecificity into a Single Albumin-Binding Domain

  title={Engineering Bispecificity into a Single Albumin-Binding Domain},
  author={Johan Nilvebrant and Tove Alm and Sophia Hober and John L{\"o}fblom},
  booktitle={PloS one},
Bispecific antibodies as well as non-immunoglobulin based bispecific affinity proteins are considered to have a very high potential in future biotherapeutic applications. In this study, we report on a novel approach for generation of extremely small bispecific proteins comprised of only a single structural domain. Binding to tumor necrosis factor-α (TNF-α) was engineered into an albumin-binding domain while still retaining the original affinity for albumin, resulting in a bispecific protein… CONTINUE READING