Engineered temperature compensation in a synthetic genetic clock

  title={Engineered temperature compensation in a synthetic genetic clock},
  author={Faiza Hussain and Chinmaya Gupta and Andrew J. Hirning and William Ott and Kathleen Shive Matthews and Kre{\vs}imir Josi{\'c} and Matthew R. Bennett},
  journal={Proceedings of the National Academy of Sciences},
  pages={972 - 977}
Significance Synthetic gene circuits are often fragile, as perturbations to cellular conditions frequently alter their behavior. This lack of robustness limits the utility of engineered gene circuits and hinders advances in synthetic biology. Here, we demonstrate that environmental sensitivity can be reduced by simultaneously engineering circuits at the protein and network levels. Specifically, we designed and constructed a synthetic genetic clock that exhibits temperature compensation—the… 

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