Engineered lactic acid bacterium Lactococcus lactis capable of binding antibodies and tumor necrosis factor alpha.

Abstract

We have optimized the display of the B domain of staphylococcal protein A on the surface of Lactococcus lactis. The maximum binding capacity was estimated at 0.146 μg of antibody per 10⁸ cells and was sustained at 86% after treatment with simulated gastric juice. A tumor necrosis factor alpha (TNF-α)-binding affibody was also displayed and bound TNF-α, which could be useful in the treatment of inflammatory bowel disease.

DOI: 10.1128/AEM.00190-10
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@article{Ravnikar2010EngineeredLA, title={Engineered lactic acid bacterium Lactococcus lactis capable of binding antibodies and tumor necrosis factor alpha.}, author={Matja{\vz} Ravnikar and B. Strukelj and Nata{\vs}a Obermajer and Mojca Lunder and Ale{\vs} Berlec}, journal={Applied and environmental microbiology}, year={2010}, volume={76 20}, pages={6928-32} }