Engineered ketol-acid reductoisomerase and alcohol dehydrogenase enable anaerobic 2-methylpropan-1-ol production at theoretical yield in Escherichia coli.

@article{Bastian2011EngineeredKR,
  title={Engineered ketol-acid reductoisomerase and alcohol dehydrogenase enable anaerobic 2-methylpropan-1-ol production at theoretical yield in Escherichia coli.},
  author={Sabine Bastian and Xiang ming Liu and Joseph T. Meyerowitz and Christopher D. Snow and Mike Yen-Yang Chen and Frances H. Arnold},
  journal={Metabolic engineering},
  year={2011},
  volume={13 3},
  pages={345-52}
}
2-methylpropan-1-ol (isobutanol) is a leading candidate biofuel for the replacement or supplementation of current fossil fuels. Recent work has demonstrated glucose to isobutanol conversion through a modified amino acid pathway in a recombinant organism. Although anaerobic conditions are required for an economically competitive process, only aerobic isobutanol production has been feasible due to an imbalance in cofactor utilization. Two of the pathway enzymes, ketol-acid reductoisomerase and… CONTINUE READING
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