Engineered holliday junctions as single-molecule reporters for protein-DNA interactions with application to a MerR-family regulator.

Abstract

Protein-DNA interactions are essential for gene maintenance, replication, and expression. Characterizing how proteins interact with and change the structure of DNA is crucial in elucidating the mechanisms of protein function. Here, we present a novel and generalizable method of using engineered DNA Holliday junctions (HJs) that contain specific protein… (More)
DOI: 10.1021/ja072485y

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