Engineered glycoforms of an antineuroblastoma IgG1 with optimized antibody-dependent cellular cytotoxic activity

@article{Umaa1999EngineeredGO,
  title={Engineered glycoforms of an antineuroblastoma IgG1 with optimized antibody-dependent cellular cytotoxic activity},
  author={Pablo Uma{\~n}a and Joel Jean-Mairet and Radmila Moudry and Hanspeter Amstutz and James Edwin Bailey},
  journal={Nature Biotechnology},
  year={1999},
  volume={17},
  pages={176-180}
}
The glycosylation pattern of chCE7, an antineuroblastoma chimeric IgG1, was engineered in Chinese hamster ovary cells with tetracycline–regulated expression of β(1,4)–N–acetylglucosaminyltransferase III (GnTIII), a glycosyltransferase catalyzing formation of bisected oligosaccharides that have been implicated in antibody–dependent cellular cytotoxicity (ADCC). Measurement of the ADCC activity of chCE7 produced at different tetracycline levels showed an optimal range of GnTIII expression for… 

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