Engineered defective interfering RNAs of Sindbis virus express bacterial chloramphenicol acetyltransferase in avian cells.

@article{Levis1987EngineeredDI,
  title={Engineered defective interfering RNAs of Sindbis virus express bacterial chloramphenicol acetyltransferase in avian cells.},
  author={Robert Levis and Henry V. Huang and Sondra Schlesinger},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1987},
  volume={84 14},
  pages={
          4811-5
        }
}
We are investigating the feasibility of using the positive-strand RNA virus Sindbis virus and its defective interfering (DI) particles as vectors for introducing foreign genes into cells. In previous work we showed by deletion mapping of a cloned cDNA derived from one of the DI RNAs that only nucleotides at the 3' and 5' termini of the RNA are essential for the DI RNA to be amplified after it is transfected into cells in the presence of helper virus. As a first step in developing a vector we… CONTINUE READING

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