Engineered allosteric ribozymes that sense the bacterial second messenger cyclic diguanosyl 5'-monophosphate.

@article{Gu2012EngineeredAR,
  title={Engineered allosteric ribozymes that sense the bacterial second messenger cyclic diguanosyl 5'-monophosphate.},
  author={Hongzhou Gu and Kazuhiro Furukawa and Ronald R. Breaker},
  journal={Analytical chemistry},
  year={2012},
  volume={84 11},
  pages={
          4935-41
        }
}
A series of allosteric ribozymes that respond to the bacterial second messenger cyclic diguanosyl-5'-monophosphate (c-di-GMP) have been created by using in vitro selection. An RNA library was generated by using random-sequence bridges to join a hammerhead self-cleaving ribozyme to an aptamer from a natural c-di-GMP riboswitch. Specific bridge sequences, called communication modules, emerged through two in vitro selection efforts that either activate or inhibit ribozyme self-cleavage upon ligand… CONTINUE READING
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