Engineered RNase P ribozymes increase their cleavage activities and efficacies in inhibiting viral gene expression in cells by enhancing the rate of cleavage and binding of the target mRNA.

@article{Zou2004EngineeredRP,
  title={Engineered RNase P ribozymes increase their cleavage activities and efficacies in inhibiting viral gene expression in cells by enhancing the rate of cleavage and binding of the target mRNA.},
  author={Hua Zou and Jarone Lee and Ahmed F Kilani and Kihoon Kim and Phong Q. Trang and Joseph B. Kim and Fenyong Liu},
  journal={The Journal of biological chemistry},
  year={2004},
  volume={279 31},
  pages={32063-70}
}
Engineered RNase P ribozymes are promising gene-targeting agents that can be used in both basic research and clinical applications. We have previously selected ribozyme variants for their activity in cleaving an mRNA substrate from a pool of ribozymes containing randomized sequences. In this study, one of the variants was used to target the mRNA encoding thymidine kinase (TK) of herpes simplex virus 1 (HSV-1). The variant exhibited enhanced cleavage and substrate binding and was at least 30… CONTINUE READING
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