Engineered CRISPR-Cas9 nucleases with altered PAM specificities

  title={Engineered CRISPR-Cas9 nucleases with altered PAM specificities},
  author={Benjamin P Kleinstiver and Michelle S. Prew and Shengdar Q Tsai and Ved V. Topkar and Nhu To Nguyen and Zongli Zheng and Andrew P. W. Gonzales and Zhuyun Li and Randall T. Peterson and Jing-Ruey Joanna Yeh and Martin J. Aryee and J. Keith Joung},
Although CRISPR-Cas9 nucleases are widely used for genome editing, the range of sequences that Cas9 can recognize is constrained by the need for a specific protospacer adjacent motif (PAM). As a result, it can often be difficult to target double-stranded breaks (DSBs) with the precision that is necessary for various genome-editing applications. The ability to engineer Cas9 derivatives with purposefully altered PAM specificities would address this limitation. Here we show that the commonly used… CONTINUE READING
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