Engineered CRISPR-Cas9 nucleases with altered PAM specificities

@inproceedings{Kleinstiver2015EngineeredCN,
  title={Engineered CRISPR-Cas9 nucleases with altered PAM specificities},
  author={Benjamin P Kleinstiver and Michelle S. Prew and Shengdar Q Tsai and Ved V. Topkar and Nhu To Nguyen and Zongli Zheng and Andrew P. W. Gonzales and Zhuyun Li and Randall T. Peterson and Jing-Ruey Joanna Yeh and Martin J. Aryee and J. Keith Joung},
  booktitle={Nature},
  year={2015}
}
Although CRISPR-Cas9 nucleases are widely used for genome editing, the range of sequences that Cas9 can recognize is constrained by the need for a specific protospacer adjacent motif (PAM). As a result, it can often be difficult to target double-stranded breaks (DSBs) with the precision that is necessary for various genome-editing applications. The ability to engineer Cas9 derivatives with purposefully altered PAM specificities would address this limitation. Here we show that the commonly used… CONTINUE READING
Highly Influential
This paper has highly influenced 31 other papers. REVIEW HIGHLY INFLUENTIAL CITATIONS
Recent Discussions
This paper has been referenced on Twitter 102 times over the past 90 days. VIEW TWEETS

Citations

Publications citing this paper.
Showing 1-10 of 322 extracted citations

References

Publications referenced by this paper.
Showing 1-10 of 35 references

Similar Papers

Loading similar papers…