Energy systems for ATP regeneration in cell-free protein synthesis reactions.

@article{Calhoun2007EnergySF,
  title={Energy systems for ATP regeneration in cell-free protein synthesis reactions.},
  author={Kara A Calhoun and James R. Swartz},
  journal={Methods in molecular biology},
  year={2007},
  volume={375},
  pages={3-17}
}
Supplying energy for cell-free protein synthesis reactions is one of the biggest challenges to the success of these systems. Oftentimes, short reaction duration is attributed to an unstable energy source. Traditional cell-free reactions use a compound with a high-energy phosphate bond, such as phosphoenolpyruvate, to generate the ATP required to drive transcription and translation. However, recent work has led to better understanding and activation of the complex metabolism that can occur… CONTINUE READING

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