Energy filtered electron tomography of ice-embedded actin and vesicles.

@article{Grimm1997EnergyFE,
  title={Energy filtered electron tomography of ice-embedded actin and vesicles.},
  author={Rudolf Grimm and M. B{\"a}rmann and Walter Haeckl and Dieter Typke and Erich. Sackmann and Wolfgang Baumeister},
  journal={Biophysical journal},
  year={1997},
  volume={72 1},
  pages={
          482-9
        }
}
Semiautomatic single-axis tilt electron tomography has been used to visualize the three-dimensional organization of actin filaments in "phantom cells," i.e. lipid vesicles. The instrumentation consisted of a 120-kV electron microscope equipped with a postcolumn energy filter, which was used in the zero-loss imaging mode. Apart from changing the tilt angle, all steps required for automated tomography, such as recentering the image area, refocusing, and centering the energy-selecting slit, were… CONTINUE READING

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