Energy-dependent quenching of dark-level chlorophyll fluorescence in intact leaves

Abstract

A new type of modulation fluorometer was used in the study of energy-dependent chlorophyll fluorescence quenching (qE) in intact leaves. Under conditions of strong energization of the thylakoid membrane (high light intensity, absence of CO2) not only variable fluorescence, FV, but also dark-level fluorescence, FO, was quenched, leading to definition of a quenching coefficient, qO. Information on qO was shown to be essential for correct determination of photochemical (qQ) and energy dependent quenching (qE) by the saturation pulse method. The relationship between qE and qO was analysed over a range of light intensities at steady state conditions. qE was found to consist of two components, the second of which is linearly correlated with qO. qO and the second component of qE are interpreted to reflect the state 1 — state 2 shift caused by LHC II phosphorylation.

DOI: 10.1007/BF00118295

3 Figures and Tables

050100150'90'93'96'99'02'05'08'11'14'17
Citations per Year

786 Citations

Semantic Scholar estimates that this publication has 786 citations based on the available data.

See our FAQ for additional information.

Cite this paper

@article{Bilger1986EnergydependentQO, title={Energy-dependent quenching of dark-level chlorophyll fluorescence in intact leaves}, author={Wolfgang Bilger and U lrich Schreiber}, journal={Photosynthesis Research}, year={1986}, volume={10}, pages={303-308} }