Energy dependent hydrogen ion accumulation in submitochondrial particles.

  title={Energy dependent hydrogen ion accumulation in submitochondrial particles.},
  author={Hagai Rottenberg and Constance Lee},
  volume={14 12},
The fluorescence quenching of 9-aminoacridine (9AA) in suspension of beef heart EDTA submitochondrial particles was studied and was used to calculate the pH gradient between these particles and the medium. This pH gradient, which is energy dependent, is also dependent strongly on the presence of anion species in the medium. It is 2.2 pH units in acetate medium and can be as high as 3.6 units in the presence of other highly lyophilic anions. The anions tested were found to be effective in the… CONTINUE READING


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