Energy-dependent changes in conformation and catalytic activity of the chloroplast ATP synthase.

@article{KomatsuTakaki1992EnergydependentCI,
  title={Energy-dependent changes in conformation and catalytic activity of the chloroplast ATP synthase.},
  author={M Komatsu-Takaki},
  journal={The Journal of biological chemistry},
  year={1992},
  volume={267 4},
  pages={2360-3}
}
Energy-dependent activation of the chloroplast ATP synthase (CF0CF1) has been elucidated by investigating the conformational changes, the ADP effect, and the catalytic cooperativity of ATP hydrolysis. Conformational change was observed by measuring the reactivity of Lys-109 of the epsilon subunit of chloroplast coupling factor 1 with pyridoxal 5'-phosphate. In the postillumination dark, the Lys-109 reactivity decreased biphasically with half-times of less than 1 and 17 s. NH4Cl accelerated the… CONTINUE READING