Energy-dependent, carrier-mediated extrusion of carboxyfluorescein from Saccharomyces cerevisiae allows rapid assessment of cell viability by flow cytometry.

Abstract

Carboxyfluorescein diacetate is a nonfluorescent compound which can be used in combination with flow cytometry for vital staining of yeasts and bacteria. The basis of this method is the assumption that, once inside the cell, carboxyfluorescein diacetate is hydrolyzed by nonspecific esterases to produce the fluorescent carboxyfluorescein (cF). cF is retained… (More)

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Cite this paper

@article{Breeuwer1994EnergydependentCE, title={Energy-dependent, carrier-mediated extrusion of carboxyfluorescein from Saccharomyces cerevisiae allows rapid assessment of cell viability by flow cytometry.}, author={Pieter Breeuwer and J L Drocourt and Frank M. Rombouts and Tjakko Abee}, journal={Applied and environmental microbiology}, year={1994}, volume={60 5}, pages={1467-72} }