Energy coupling in bacterial periplasmic transport systems. Studies in intact Escherichia coli cells.

@article{Joshi1989EnergyCI,
  title={Energy coupling in bacterial periplasmic transport systems. Studies in intact Escherichia coli cells.},
  author={Aravind K. Joshi and S Ahmed and G Ferro-Luzzi Ames},
  journal={The Journal of biological chemistry},
  year={1989},
  volume={264 4},
  pages={2126-33}
}
Periplasmic permeases are composed of four proteins, one of which has an ATP-binding site that has been postulated to be involved in energy coupling. Previous data suggested that these permeases derive energy from substrate level phosphorylation (Berger, E. A. (1973) Proc. Natl. Acad. Sci. U.S.A. 70, 1514-1518); however, conflicting results later cast doubt upon this hypothesis. Here, we make use of two well characterized periplasmic permeases and of a well characterized unc mutant (ATPase-) to… CONTINUE READING