Energetics of a hydrogen bond (charged and neutral) and of a cation-pi interaction in apoflavodoxin.

@article{FernndezRecio1999EnergeticsOA,
  title={Energetics of a hydrogen bond (charged and neutral) and of a cation-pi interaction in apoflavodoxin.},
  author={Juan Fern{\'a}ndez-Recio and Antonio Romero and Javier Sancho},
  journal={Journal of molecular biology},
  year={1999},
  volume={290 1},
  pages={
          319-30
        }
}
Anabaena apoflavodoxin contains a single histidine residue (H34) that interacts with two aromatic residues (F7 and Y47). The histidine and phenylalanine rings are almost coplanar and they can establish a cation-pi interaction when the histidine is protonated. The histidine and tyrosine side-chains are engaged in a hydrogen bond, which is their only contact. We analyse the energetics of these interactions using p Ka-shift analysis, double-mutant cycle analysis at two pH values, and X-ray… 
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