Energetics of DNA twisting. II. Topoisomer analysis.

  title={Energetics of DNA twisting. II. Topoisomer analysis.},
  author={David Shore and Robert L. Baldwin},
  journal={Journal of molecular biology},
  volume={170 4},
A gel electrophoresis method has been developed for resolving small (approximately equal to 250 bp DNA topoisomers. In this size range only one major topoisomer band is observed, except for ligase closure conditions in which the probabilities are nearly equal for circularization by untwisting and overtwisting the corresponding linear DNA. The two probabilities are nearly equal when delta Tw is close to 0.5, if the mean helical twist of the linear DNA is n + delta Tw, where n is an integer and… CONTINUE READING