• Corpus ID: 42797350

EndothelinA Receptor Subtype Mediates Endothelin-Induced Contractility in Left Ventricular Cardiomyocytes Isolated from Rabbit Myocardium 1

@inproceedings{Kelso2000EndothelinARS,
  title={EndothelinA Receptor Subtype Mediates Endothelin-Induced Contractility in Left Ventricular Cardiomyocytes Isolated from Rabbit Myocardium 1},
  author={Elizabeth J. Kelso and Barbara J. McDermott and Bernard Silke and James Paul Spiers},
  year={2000}
}
Endothelin (ET)-1 is a potent positive inotropic agent, the effects of which are mediated by increases in cytosolic Ca in the myocardium. The object of this study was to examine 1) the influence of ETA and ETB receptor subtypes, and 2) the role of the phospholipase C (PLC) pathway in mediating ET-1-induced contraction. Left ventricular cardiomyocytes were isolated from the hearts of New Zealand White rabbits (2–2.5 kg) by the use of Langendorff perfusion with collagenase. Cardiomyocyte function… 

Figures from this paper

References

SHOWING 1-10 OF 33 REFERENCES
Endothelin has potent direct inotropic and chronotropic effects in cultured heart cells.
TLDR
The results indicate that endothelin-1 is one of the most potent inotropic agents in cultured cardiac myocytes, and suggest that this effect involves, at least in part, a calcium ion influx through voltage-sensitive calcium channels.
Endothelin enhances the contractile responsiveness of adult rat ventricular myocytes to calcium by a pertussis toxin-sensitive pathway.
TLDR
The novel and potent pharmacologic action of endothelin points to the potential importance of local, paracrine factors, perhaps derived from microvascular endothelium or endocardium, in the control of the contractile function of the heart.
Endothelin is a positive inotropic agent in human and rat heart in vitro.
Pharmacological properties of endothelin receptor subtypes mediating positive inotropic effects in rabbit heart.
TLDR
Findings indicate that the PIE of ET receptor agonists on rabbit ventricular myocardium cannot be totally explained by occupancy of the ETA or ETB receptor.
Exogenous effects and endogenous production of endothelin in cardiac myocytes: potential significance in heart failure.
TLDR
It is suggested that the production of ET may have important and potentially differential effects on contractile function with the development of CHF.
Positive inotropic action of novel vasoconstrictor peptide endothelin on guinea pig atria.
TLDR
The results suggest that the ET-induced response on the left atria is intimately related to the influx of extracellular Ca2+.
Endothelin and increased contractility in adult rat ventricular myocytes. Role of intracellular alkalosis induced by activation of the protein kinase C-dependent Na(+)-H+ exchanger.
TLDR
The positive inotropic action of endothelin is due in part to stimulation of the sarcolemmal Na(+)-H+ exchanger by a protein kinase C-mediated pathway, resulting in a rise in pHi and sensitization of cardiac myofilaments to intracellular Ca2+.
Myocardial contractile actions of endothelin-1 in rat and rabbit papillary muscles. Role of endocardial endothelium.
TLDR
It would appear that endothelin increases contractility and modifies twitch configuration in rat and rabbit papillary muscles and in a species, such as rabbit, that responds more to interventions that increase intracellular calcium.
Characteristics of binding of endothelin-1 and endothelin-3 to rat hearts. Developmental changes in mechanical responses and receptor subtypes.
TLDR
Endothelin-1 (ET-1) and ET-3 produced positive inotropic effects on electrically stimulated left atria and increased the frequency of spontaneously beating right atria of adult rats and caused marked positive inotropy in the neonatal ventricles.
...
...