Endothelial cells derived from human embryonic stem cells form durable blood vessels in vivo

@article{Wang2007EndothelialCD,
  title={Endothelial cells derived from human embryonic stem cells form durable blood vessels in vivo},
  author={Zack Z Wang and Patrick Au and Tong Chen and Ying Shao and Laurence Daheron and Hao Bai and Melanie Arzigian and Dai Fukumura and Rakesh K. Jain and David T. Scadden},
  journal={Nature Biotechnology},
  year={2007},
  volume={25},
  pages={317-318}
}
We describe the differentiation of human embryonic stem (hES) cells into endothelial cells using a scalable two-dimensional method that avoids an embryoid-body intermediate. After transplantation into severe combined immunodeficient (SCID) mice, the differentiated cells contributed to arborized blood vessels that integrated into the host circulatory system and served as blood conduits for 150 d. 

Endothelial differentiation of embryonic stem cells.

Methods for isolating purified proliferating populations of vascular endothelial cells from mouse embryonic stem cells from mESC using Flk-1 positive sorted cells, VEGF supplementation, and a rigorous manual selection technique required for endothelial cell purification and expansion are described.

Building blood vessels—stem cell models in vascular biology

This review examines vasculogenesis and angiogenesis in murine embryoid bodies and discusses the promise of stem cell–based models for the study of human vascular development.

Directing human embryonic stem cells to generate vascular progenitor cells

The current status of directing hESCs to differentiate to vascular cells is reviewed and it is suggested that endothelial cells and SMCs derived from h ESCs can be used to engineer artificial vessels to repair damaged vessels and form vessel networks in engineered tissues.

Vascular Differentiation of Human Pluripotent Stem Cells

This chapter reviews the progresses done so far in the vascular differentiation of hPSCs focusing in the efficiency and kinetics of the differentiation protocols, inductive signals, maturation, and functionality of the hPSC-derived cells and their therapeutic potential.

Embryonic stem cell models in vascular biology

Through culture of mouse embryonic stem cell, it is discovered that differentiating stem cells are highly amenable to analyzing biochemical and cell biologic processes that are independent of flow.

Directed Endothelial Progenitor Differentiation from Human Pluripotent Stem Cells Via Wnt Activation Under Defined Conditions.

A 10-day, two-stage process that recapitulates endothelial cell development, in which hPSCs first differentiate to endothelial progenitors that then generate functional endothelial cells and smooth muscle cells is demonstrated.

Development of Hematopoietic and Endothelial Cells from Human Embryonic Stem Cells: Lessons from the Studies using Mouse as a Model

The latest advances using the hESC model system are focused on, capitalizing on the well-established mouse embryonic stem cell model system, as a means to investigate the lineage commitment events underlying the early embryonic development of human hematopoietic and endothelial cells.

Engineering tissue from human embryonic stem cells

Stem cell tissue engineering investigates the role of Ectodermal tissues in the regeneration of connective tissue and its role in wound healing.

Generation of vascular endothelial and smooth muscle cells from human pluripotent stem cells

This work found that GSK3 inhibition and BMP4 treatment rapidly committed pluripotent cells to a mesodermal fate and subsequent exposure to VEGF-A or PDGF-BB resulted in the differentiation of either endothelial or vascular smooth muscle cells, respectively.

Vascular diseases await translation of blood vessels engineered from stem cells

Current developments in the generation of vascular progenitor cells from hiPSCs and the assessment of their functional capacity in vivo are discussed, opportunities and challenges for the clinical translation of engineered vascular tissue, and modeling of vascular diseases using hiPSC-derived vascular progentitor cells are discussed.
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