Endothelial‐Dependent Relaxation Induced by Leukotrienes C4, D4, and E4 in Isolated Canine Arteries

  title={Endothelial‐Dependent Relaxation Induced by Leukotrienes C4, D4, and E4 in Isolated Canine Arteries},
  author={Roberta J. Secrest and Barry M. Chapnick},
  journal={Circulation Research},
Leukotriene D4 has been shown to possess the capacity to relax canine superior mesenteric and renal arterial rings in an endothelial-dependent manner. The present study was designed to determine if the remaining peptidoleukotrienes, leukotrienes C4 and E4, share this property. In addition, influences of atropine and of inhibitors of cyclooxygenase and lipoxygenase activities on relaxation produced by leukotriene D4 and acetylcholine were determined to characterize further leukotriene D4-induced… Expand
Leukotrienes C4 and D4 are potent endothelium-dependent relaxing agents in canine splanchnic venous capacitance vessels.
The peptide LTs, the major components of the slow-reacting substance of anaphylaxis, exert a profound endothelium-dependent relaxant effect on venous capacitance vessels, which is only partially dependent on L-arginine and nitric oxide. Expand
Vascular Responses to Leukocyte Products in Atherosclerotic Primates
Little is known about the possible role of leukocytes in the pathogenesis of vasospasm. We hypothesized that vasoactive products released by leukocytes might produce constriction of atheroscleroticExpand
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The leukotrienes, either through direct effects on vessels or through recruitment of inflammatory cells, are likely contributors to the nonhydrostatic edema associated with these syndromes. Expand
Nitric oxide-generating vasodilators and 8-bromo-cyclic guanosine monophosphate inhibit mitogenesis and proliferation of cultured rat vascular smooth muscle cells.
Results suggest that endogenous nitric oxide may function as a modulator of vascular smooth muscle cell mitogenesis and proliferation, by a cGMP-mediated mechanism. Expand
Inhibitory effect of ammonium chloride on acetylcholine-induced relaxation.
Intracellular alkalinization by ammonium chloride attenuated acetylcholine-induced relaxation, possibly through the interrelated production of both thromboxane A2 and superoxide radicals. Expand
Coronary reperfusion in dogs inhibits endothelium-dependent relaxation: role of superoxide radicals.
Previous studies indicate that release of superoxide radicals during coronary reperfusion following occlusion may relate to the loss of endothelium-dependent coronary arterial relaxation. We examinedExpand
Effects of thrombosis on vascular tone in rat mesenteric arteries with endothelium in vivo.
It is concluded that a partially occlusive thrombus may release a material that inhibits vascular constriction by serotonin or norepinephrine through an endothelium-dependent mechanism and that an occlusion induces constriction of the downstream vascular bed even with endothelia. Expand
Metabolism of arachidonic acid by canine polymorphonuclear leukocytes synthesis of lipoxygenase and omega-oxidized metabolites.
Arachidonic acid is metabolized in canine PMNs through the cyclooxygenase, lipoxygenases and cytochrome P-450 pathways, and whether these metabolites contribute to myocardial injury remains to be determined. Expand
Studies on molecular properties and functional regulation of terminal leukotriene C4 synthases and cysteinyl-leukotriene receptor signalling in human endothelium
The transformation of the unstable intermediate leukotriene (LT) A4 into the glutathione conjugate LTC4, the parent compound of LTD4 and LTE4, is catalysed by leukotriene C4 synthase (LTC4S) as wellExpand
International Union of Pharmacology XXXVII. Nomenclature for Leukotriene and Lipoxin Receptors
The aim of this review is to provide the molecular evidence as well as the properties and significance of the leukotriene and lipoxin receptors, which has lead to the present nomenclature. Expand


Leukotriene D4 relaxes canine renal and superior mesenteric arteries.
It is demonstrated that leukotriene D4 possesses the capacity to relax canine superior mesenteric and renal arterial rings in an endothelial-dependent manner. Expand
Contractile activities of leukotrienes C4 and D4 on vascular strips from rabbits.
Results indicate that the leukotrienes may act as potent, selective coronary vasoconstrictors and that SRS-A responsive receptors exist in the rabbit coronary artery and that LTD4 has a direct effect on the coronary arteries. Expand
Differential effects of leukotrienes C4, D4 and E4 in the canine renal and mesenteric vascular beds.
  • L. Feigen
  • Medicine
  • The Journal of pharmacology and experimental therapeutics
  • 1983
Effects of close-arterial injections of synthetic LTs C4, D4 and E4 were determined in the renal and superior mesenteric vascular beds of anesthetized dogs, suggesting that endogenous prostaglandin synthesis did not mediate or modulate responses to the LTs. Expand
Endothelium-derived relaxant factor inhibits effects of nitrocompounds in isolated arteries.
EDRF attenuates the arterial vasodilation induced by SNP and Teopranitol, and suggests that endothelial cells exhibit a greater basal release of EDRF in the femoral artery than in the aorta, since under unstimulated conditions an EDRf-induced attenuation was seen only in femoral and not in aortic segments. Expand
Vasoconstrictor effects of leukotrienes C4 and D4 in the feline mesenteric vascular bed.
The present data show that LTC4 and LTD4 possess significant vasoconstrictor activity in the feline mesenteric vascular bed and suggest that products of the cyclooxygenase pathway do not mediate vasoconStrictor responses to LTC 4 and LTD 4 in the intestinal circulation of the cat. Expand
Effects of the leukotrienes on the vasculature and blood pressure of different species.
It is demonstrated that the distal segment of the guinea-pig pulmonary artery is a useful and convenient preparation for the study of LT vascular pharmacology, and in vitro, LTs had no effect on aortic rings from rats, rabbits or guinea pigs. Expand
Divergent influences of leukotrienes C4, D4, and E4 on mesenteric and renal blood flow.
  • B. Chapnick
  • Biology, Medicine
  • The American journal of physiology
  • 1984
The results demonstrate that LTC4, LTD4, and LTE4 differentially affect blood flow in the intestine and kidney and suggest that if circulating levels of leukotrienes are increased, blood flow would be expected to be diverted away from the intestine. Expand
Leukotrienes C4, D4 and E4: effects on human and guinea-pig cardiac preparations in vitro.
The cardiac effects of pure synthetic LT are similar to those previously obtained with crude slow-reacting substance of anaphylaxis indicating that the prolonged contractile failure associated with systemic anAPHylaxis largely could be due to the negative inotropic effect of LT. Expand
C-6-sulfidopeptide leukotrienes are unlikely to be involved in the endothelium dependent relaxation of rabbit aorta by acetylcholine.
It is concluded from data, that C-6-sulfidopeptide leukotrienes, although probably produced by vascular tissue, are unlikely to be involved in the ACh-induced relaxation of rabbit aorta. Expand
Cytochrome P-450-dependent monooxygenase activity and endothelial-dependent relaxations induced by arachidonic acid.
SKF 525A, an inhibitor of cytochrome P-450-dependent monooxygenase, markedly inhibits AA-induced relaxations in intact rings in a dose-dependent manner while not affecting the response in rings denuded of endothelium. Expand