Endoribonuclease RNase III is essential in Bacillus subtilis.

  title={Endoribonuclease RNase III is essential in Bacillus subtilis.},
  author={M A Herskovitz and David H. Bechhofer},
  journal={Molecular microbiology},
  volume={38 5},
The rncS gene of Bacillus subtilis encodes Bs-RNase III, a narrow-specificity endoribonuclease. Previous attempts to disrupt rncS were unsuccessful. Here, a strain was constructed in which Bs-RNase III expression was dependent upon transcription of rncS from a temperature-sensitive plasmid. Growth of this strain at the non-permissive temperature resulted in 90-95% cell death, and virtually all the cells that survived retained the rncS-expressing plasmid. Thus, we conclude that rncS is essential… CONTINUE READING


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