Endophytic fungi found in association with Smallanthus sonchifolius (Asteraceae) as resourceful producers of cytotoxic bioactive natural products.

Abstract

Smallanthus sonchifolius is a traditional Andean plant which has been cultured mainly in Brazil, Japan and New Zealand due to its medicinal properties. A study of the endophytic fungi associated to the plant was carried out in order to characterize new cytotoxic agents. Thirty two fungal strains were isolated and submitted to cultivation and extraction producing 186 extracts. Of these, 12% displayed moderate to high cytotoxic activities and were considered promising anticancer compound sources. The ethyl acetate fractions of Nigrospora sphaerica and Phoma betae liquid fermentations contained the synergistic compounds 8-hydroxy-6-methoxy-3-methylisocoumarin and (22E,24R)-ergosta-4,6,8(14),22-tetraen-3-one which are potential compounds for drug discovery. Another isolated compound, pimara-7,15-dien-3-beta-ol diterpene is being characterized for the first time through a detailed spectroscopic analysis including GC/MS, homo- and hetero-nuclear correlated NMR experiments (HMQC, HMBC, COSY and NOEdiff) along with its optical rotation.

DOI: 10.1002/jobm.200800093

Cite this paper

@article{Gallo2009EndophyticFF, title={Endophytic fungi found in association with Smallanthus sonchifolius (Asteraceae) as resourceful producers of cytotoxic bioactive natural products.}, author={Margareth Borges Coutinho Gallo and Fernanda Oliveira Chagas and Mar{\'i}lia O. Almeida and Claudia de Assis Corr{\^e}a de Macedo and Bruno Co{\^e}lho Cavalcanti and Francisco Washington Ara{\'u}jo Barros and Manoel Odor{\'i}co de Moraes and Let{\'i}cia V Costa-Lotufo and Cl{\'a}udia do {\'O}. Pessoa and Jairo Kenupp Bastos and M{\^o}nica Tallarico Pupo}, journal={Journal of basic microbiology}, year={2009}, volume={49 2}, pages={142-51} }