Endonuclease activities associated with high molecular weight and internucleosomal DNA fragmentation in apoptosis.

@article{Walker1994EndonucleaseAA,
  title={Endonuclease activities associated with high molecular weight and internucleosomal DNA fragmentation in apoptosis.},
  author={Peter F R Walker and Valerie M Weaver and Boleslaw Lach and Julie Leblanc and Marianna Sikorska},
  journal={Experimental cell research},
  year={1994},
  volume={213 1},
  pages={100-6}
}
We have used pulsed-field gel electrophoresis and electron microscopy to correlate stages of DNA fragmentation with alterations of nuclear structure during apoptosis. DNA fragmentation occurs in two stages. The first is initiated by a previously undescribed endonucleolytic activity that cleaves DNA into 50- to 300-kb fragments. Electron microscopy showed that this degree of cleavage was sufficient to cause the chromatin to undergo condensation. The second stage of fragmentation is catalyzed by… CONTINUE READING

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