Endogenous versus exogenous thiols in radioprotection.

  title={Endogenous versus exogenous thiols in radioprotection.},
  author={O. Vos and W S Roos-Verhey},
  journal={Pharmacology \& therapeutics},
  volume={39 1-3},

Radiation Oncology: Past Achievements

Major advances in cancer therapy are likely to come with improved combined modality treatment representing inte gration of local modalities with the systemic, according to this opinion.

Glutathione and glutathione delivery compounds.

Effects of cadmium on haemopoiesis in irradiated and non-irradiated mice: 1. Relationship to the number of myeloid progenitor cells.

Pretreatment with cadmium significantly reduced the lethal effects of gamma rays and increased the number of endogenous haemopoietic stem cells (endoCFU-S) in a concentration-dependent manner.

Radioprotective effect of exogenous glutathione on rat parotid glands.

It was shown that elevation of the intraglandular level of NPSH after exogenous administration of GSH protected the parotid glands from radiation injury in the rat.

Thiol uptake by Chinese hamster V79 cells and aerobic radioprotection as a function of the net charge on the thiol.

Thiols studied showed that hydroxyl radical scavenging and DNA radical repair are important mechanisms in the protection of cells by thiols and that the net charge on the thiol is a significant factor in its effectiveness.

Glutathione modulation in cancer treatment: will it work?



The fate of extracellular glutathione in the rat.

Protection against x-irradiation by sulphdryl compounds. I. Studies on the cellular uptake under various conditions of incubation.

  • H. ZwenkA. VergroesenO. Vos
  • Biology
    International journal of radiation biology and related studies in physics, chemistry, and medicine
  • 1967
Data obtained in experiments with 35S-labelled cysteamine, cystamines, cysteine, thioglycol and glutathione showed that these compounds may not be taken up to the same extent by tissue culture cells.

Intracellular cysteine delivery system that protects against toxicity by promoting glutathione synthesis.

An intracellular cysteine delivery system was used to promote glutathione synthesis, and this was found to protect against toxicity, and the thiazolidine, which is converted to L-cysteine by the enzyme 5-oxo-L-prolinase, is the actual protectant.

Equilibrium dialysis studies of the binding of radioprotector compounds to DNA.

Results indicate that binding of WR-33278 and WR-1065 by DNA phosphate are probably significant in the mechanism of radioprotection by WR-2721 and cysteamine and cystamine bound to DNA with comparable affinity under similar conditions.

Lack of oxygen effect in glutathione-deficient human cells in culture.

The frequency of X-ray-induced DNA breaks was determined in human cell lines which are deficient in glutathione synthetase and have a greatly reduced glutathion content, and the dose-effect relationship for the induction of breaks when radiation exposure was made in argon, was similar to that found when exposure wasmade in air.

Studies on the relationship between the radiation resistance and glutathione content of human and rodent cells after treatment with dexamethasone in vitro.

  • B. C. MillarS. Jinks
  • Medicine, Biology
    International journal of radiation biology and related studies in physics, chemistry, and medicine
  • 1985
The results suggest that enhancement of radiation resistance cannot be directly ascribed to an elevated GSH content in steroid-treated cells, and it is unlikely that the efficacy of radiotherapy will be diminished amongst patients receiving concomitant treatment with dexamethasone.

A pulse radiolytic study of the interaction of nitroxyls with free-radical adducts of purines: consequences for radiosensitization.

  • P. O'NeillS. Davies
  • Chemistry
    International journal of radiation biology and related studies in physics, chemistry, and medicine
  • 1986
It has been confirmed that the properties of the radical produced on interaction of Br2-. with dGMP, based upon its subsequent interactions with nitroxyls, are quantitatively the same as those for the .OH-radical adduct of dG MP with oxidizing properties.