Endogenous glucocorticoid analysis by liquid chromatography–tandem mass spectrometry in routine clinical laboratories

  title={Endogenous glucocorticoid analysis by liquid chromatography–tandem mass spectrometry in routine clinical laboratories},
  author={James M. Hawley and Brian G Keevil},
  journal={The Journal of Steroid Biochemistry and Molecular Biology},
  • J. Hawley, B. Keevil
  • Published 1 September 2016
  • Biology
  • The Journal of Steroid Biochemistry and Molecular Biology
Quantitative MALDI-MS/MS assay for serum cortisol through charged derivatization.
The evolution of methods for urinary steroid metabolomics in clinical investigations particularly in childhood
Trends in Analysis of Cortisol and Its Derivatives.
The literature on the MS and non-MS based methods for determination of cortisol and its derivatives are reviewed and the use of a less used quadrupole-time of flight instrument is explored in determination of these compounds.
20α- and 20β-dihydrocortisone may interfere in LC-MS/MS determination of cortisol in saliva and urine
Cortisol determination by LC-MS/MS may suffer from erroneously elevated results unless 20α- and 20β-dihydrocortisone are chromatographically separated from cortisol.
LC-MSMS assays of urinary cortisol, a comparison between four in-house assays
Compared UFC results using four LC-MSMS methods performed in four independent laboratories in order to evaluate interlaboratory agreement, variations of results can be found depending on analytical process.


Steroid hormone analysis by tandem mass spectrometry.
LC-MS/MS now affords the specificity, imprecision, and limits of quantification necessary for the reliable measurement of steroids in human fluids, enhancing diagnostic capabilities, particularly when steroid profiles are available.
Novel liquid chromatography tandem mass spectrometry (LC-MS/MS) methods for measuring steroids.
  • B. Keevil
  • Biology, Chemistry
    Best practice & research. Clinical endocrinology & metabolism
  • 2013
Multisteroid LC–MS/MS assay for glucocorticoids and androgens and its application in Addison's disease
The data suggest that women with AD are particularly androgen deficient after menopause, and this automated, high-throughput LC–MS/MS assay provides highly sensitive and specific assessments of glucocorticoids and androgens with low sample volumes and is suitable for endocrine laboratories and research.
LC-MS/MS in the Clinical Laboratory - Where to From Here?
While sample throughput is higher than for conventional HPLC or GC-MS, it lags behind automated immunoassays, and measures to improve specificity and sensitivity include sample clean-up and optimising chromatography to avoid interferences and ion suppression due to sample-matrix components.
Liquid chromatography-tandem mass spectrometry analysis of urinary free cortisol.
A direct sample injection method similar to one published by Nassar et al. (2) is developed, using a guard cartridge for trapping cortisol as part of an online purification strategy and the use of step gradient rather than isocratic LC conditions.
Pitfalls associated with the use of liquid chromatography-tandem mass spectrometry in the clinical laboratory.
LC-MS/MS methods should undergo rigorous and systematic validation before introduction into patient care and can be controlled by sufficient LC separation-based sample workup before MS analysis.
Quantitative analysis of hydrocortisone in human urine using a high-performance liquid chromatographic-tandem mass spectrometric-atmospheric-pressure chemical ionization method.
Validation parameters such as specificity/selectivity, limit of quantitation (LOQ), linearity, precision, accuracy, ruggedness, stability, and system suitability are evaluated for this HPLC-MS-MS method.
Improved liquid chromatography–tandem mass spectrometry method in clinical utility for the diagnosis of Cushing’s syndrome
An improved liquid chromatography–tandem mass spectrometric (LC–MS/MS) method was developed for the simultaneous determination of urinary free cortisol and cortisone and has shown superior analytical advantages over existing immunoassay type in terms of sensitivity, specificity and capability to diagnose Cushing's syndrome.