Endogenous glucocorticoid analysis by liquid chromatography–tandem mass spectrometry in routine clinical laboratories

@article{Hawley2016EndogenousGA,
  title={Endogenous glucocorticoid analysis by liquid chromatography–tandem mass spectrometry in routine clinical laboratories},
  author={James M. Hawley and Brian G Keevil},
  journal={The Journal of Steroid Biochemistry and Molecular Biology},
  year={2016},
  volume={162},
  pages={27-40}
}
  • J. Hawley, B. Keevil
  • Published 1 September 2016
  • Biology
  • The Journal of Steroid Biochemistry and Molecular Biology
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The literature on the MS and non-MS based methods for determination of cortisol and its derivatives are reviewed and the use of a less used quadrupole-time of flight instrument is explored in determination of these compounds.
20α- and 20β-dihydrocortisone may interfere in LC-MS/MS determination of cortisol in saliva and urine
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Cortisol determination by LC-MS/MS may suffer from erroneously elevated results unless 20α- and 20β-dihydrocortisone are chromatographically separated from cortisol.
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Compared UFC results using four LC-MSMS methods performed in four independent laboratories in order to evaluate interlaboratory agreement, variations of results can be found depending on analytical process.
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