Endogenous channels in HEK cells and potential roles in HCN ionic current measurements.

@article{Varghese2006EndogenousCI,
  title={Endogenous channels in HEK cells and potential roles in HCN ionic current measurements.},
  author={Anthony Varghese and Erica M Tenbroek and James Coles and Daniel C. Sigg},
  journal={Progress in biophysics and molecular biology},
  year={2006},
  volume={90 1-3},
  pages={26-37}
}
A transformed line of human embryonic kidney epithelial cells (HEK 293) is commonly used as an expression system for exogenous ion channel genes. Previously, it has been shown that these cells contain mRNAs for a variety of ion channels. Expression of some of these genes has been confirmed at the protein level. Patch-clamp electrophysiology experiments confirm the presence of multiple ion channels and molecular data agree with pharmacological profiles of identified channels. In this work, we… CONTINUE READING