Endogenous and endobiotic induced reactive oxygen species formation by isolated hepatocytes.

@article{Siraki2002EndogenousAE,
  title={Endogenous and endobiotic induced reactive oxygen species formation by isolated hepatocytes.},
  author={Arno G. Siraki and Jalal Pourahmad and Tom S. Chan and Sumsullah Khan and Peter James O'Brien},
  journal={Free radical biology & medicine},
  year={2002},
  volume={32 1},
  pages={
          2-10
        }
}
The rat hepatocyte catalyzed oxidation of 2',7'-dichlorofluorescin to form the fluorescent 2,7'-dichlorofluorescein was used to measure endogenous and xenobiotic-induced reactive oxygen species (ROS) formation by intact isolated rat hepatocytes. Various oxidase substrates and inhibitors were then used to identify the intracellular oxidases responsible. Endogenous ROS formation was markedly increased in catalase-inhibited or GSH-depleted hepatocytes, and was inhibited by ROS scavengers or… CONTINUE READING
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