Endocytic protein intersectin-l regulates actin assembly via Cdc42 and N-WASP

@article{Hussain2001EndocyticPI,
  title={Endocytic protein intersectin-l regulates actin assembly via Cdc42 and N-WASP},
  author={Natasha K. Hussain and Sarah Jenna and Michael Glogauer and Christopher C. Quinn and Sylwia Wasiak and Michel Guipponi and Stylianos E. Antonarakis and Brian K. Kay and Thomas Peter Stossel and Nathalie Lamarche-Vane and Peter S. McPherson},
  journal={Nature Cell Biology},
  year={2001},
  volume={3},
  pages={927-932}
}
Intersectin-s is a modular scaffolding protein regulating the formation of clathrin-coated vesicles. In addition to the Eps15 homology (EH) and Src homology 3 (SH3) domains of intersectin-s, the neuronal variant (intersectin-l) also has Dbl homology (DH), pleckstrin homology (PH) and C2 domains. We now show that intersectin-l functions through its DH domain as a guanine nucleotide exchange factor (GEF) for Cdc42. In cultured cells, expression of DH-domain-containing constructs cause actin… 
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Cdc42 and Ras Cooperate to Mediate Cellular Transformation by Intersectin-L*
TLDR
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TLDR
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TLDR
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TLDR
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Intersectin‐1L nucleotide exchange factor regulates secretory granule exocytosis by activating Cdc42
TLDR
The results support the idea that intersectin‐1L is an adaptor that coordinates exo–endocytotic membrane trafficking in secretory cells and extend the current role of intersectin-1L in endocytosis to a function in exocythesis.
The Minimal Autoinhibited Unit of the Guanine Nucleotide Exchange Factor Intersectin
TLDR
It is proposed that the actual autoinhibited structure contains the related intramolecular SH3(E)-DH interaction, which may block or distort the GTPase binding region of the DH domain.
Phosphorylation of IQGAP1 Modulates Its Binding to Cdc42, Revealing a New Type of Rho-GTPase Regulator*
TLDR
It is proposed that, depending on its phosphorylation state, IQGAP1 might serve as an effector or sequester nucleotide-free Cdc42 to prevent signaling and uncovers a novel type of Rho-GTPase regulator.
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References

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TLDR
Immunofluorescence analysis of cultured hippocampal neurons and antibodies against intersectin co-immunoprecipitate clathrin, AP2, and dynamin from brain extracts suggest that the long and short forms of intersectin are components of the endocytic machinery in neurons and nonneuronal cells.
CdGAP, a Novel Proline-rich GTPase-activating Protein for Cdc42 and Rac*
TLDR
A novel serine- and proline-rich GTPase-activating protein, CdGAP, which is active in vitro on both Cdc42 and Rac and likely to participate in CDC42- and Rac-induced signaling pathways leading to actin reorganization is identified.
Yeast Eps15-like endocytic protein, Pan1p, activates the Arp2/3 complex
TLDR
It is suggested that Pan1p forms the core of an endocytic complex and physically couples actin polymerization nucleated by the Arp2/3 complex to the endocytical machinery, thus providing the forces necessary for endocytosis.
N‐WASP, a novel actin‐depolymerizing protein, regulates the cortical cytoskeletal rearrangement in a PIP2‐dependent manner downstream of tyrosine kinases.
TLDR
It is concluded that N‐WASP transmits signals from tyrosine kinases to cause a polarized rearrangement of cortical actin filaments dependent on PIP2.
The endocytic protein intersectin is a major binding partner for the Ras exchange factor mSos1 in rat brain
TLDR
The discovery of a brain‐enriched, 170 kDa protein that interacts specifically with SH3A suggests that intersectin functions in cell signaling in addition to its role in endocytosis and may link these cellular processes.
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TLDR
It is demonstrated that before it can induce filopodium formation, Cdc42 must bind a WASP-related protein, N-WASP, that is richest in neural tissues but is expressed ubiquitously.
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TLDR
A permeabilization method that retains coupling between N-formyl-methionyl-leucyl-phenylalanine tripeptide (FMLP) receptor stimulation, shape changes, and barbed-end actin nucleation in human neutrophils is developed and concludes that this alternate involves phosphoinositide-mediated uncapping of actin filament barbed ends.
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TLDR
The efficient reconstitution of ATP-, GTP-, cytosol- and dynamin-dependent formation of clathrin-coated vesicles in permeabilized 3T3-L1 cells is reported, suggesting that interactions between SH3 domains and their partners function sequentially in endocytic coated-vesicle formation.
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TLDR
It is shown that Eps8 and E3b1/Abi-1 participate in the transduction of signals from Ras to Rac, by regulating Rac-specific guanine nucleotide exchange factor (GEF) activities.
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