Endocytic protein intersectin-l regulates actin assembly via Cdc42 and N-WASP

@article{Hussain2001EndocyticPI,
  title={Endocytic protein intersectin-l regulates actin assembly via Cdc42 and N-WASP},
  author={Natasha K. Hussain and Sarah Jenna and Michael Glogauer and Christopher C. Quinn and Sylwia Wasiak and Michel Guipponi and Stylianos E. Antonarakis and Brian K. Kay and Thomas Peter Stossel and Nathalie Lamarche-Vane and Peter S. McPherson},
  journal={Nature Cell Biology},
  year={2001},
  volume={3},
  pages={927-932}
}
Intersectin-s is a modular scaffolding protein regulating the formation of clathrin-coated vesicles. In addition to the Eps15 homology (EH) and Src homology 3 (SH3) domains of intersectin-s, the neuronal variant (intersectin-l) also has Dbl homology (DH), pleckstrin homology (PH) and C2 domains. We now show that intersectin-l functions through its DH domain as a guanine nucleotide exchange factor (GEF) for Cdc42. In cultured cells, expression of DH-domain-containing constructs cause actin… 
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TLDR
ITSN-2L, a guanine nucleotide exchange factor for Cdc42, regulates different steps of caveola endocytosis in ECs by controlling the temporal and spatial actin polymerization and remodeling sub-adjacent to the plasma membrane.
Cdc42 and Ras Cooperate to Mediate Cellular Transformation by Intersectin-L*
TLDR
The effects of expressing different forms of the Cdc42-specific guanine nucleotide exchange factor, intersectin-L, in fibroblasts found that SH3A-C2 exhibited strong transforming activity through its ability to mediate cooperation between Ras and CDC42.
Intersectin 1 forms complexes with SGIP1 and Reps1 in clathrin-coated pits.
The Activity of the GTPase-activating Protein CdGAP Is Regulated by the Endocytic Protein Intersectin*
TLDR
Observations provide the first demonstration of a direct regulation of RhoGAP activity through a protein-protein interaction and suggest a function for intersectin in Rac1 regulation and actin dynamics.
IQGAP1 Stimulates Actin Assembly through the N-Wasp-Arp2/3 Pathway*
TLDR
It is proposed that signal-induced relief of the autoinhibited fold of IQGAP1 allows activation of N-WASP to stimulate Arp2/3-dependent actin assembly, and co-immunoprecipitation supports an in vivo link between IQG AP1 and N- WASP.
Intersectin goes nuclear: secret life of an endocytic protein.
TLDR
ITSN1-s is an endocytic protein, which shuttles between the nucleus and the cytoplasm in a CRM-1- and IMPα-dependent fashion, and is identified as a nucleocy toplasmic trafficking protein.
Intersectin‐1L nucleotide exchange factor regulates secretory granule exocytosis by activating Cdc42
TLDR
The results support the idea that intersectin‐1L is an adaptor that coordinates exo–endocytotic membrane trafficking in secretory cells and extend the current role of intersectin-1L in endocytosis to a function in exocythesis.
Tuba, a GEF for CDC42, links dynamin to actin regulatory proteins.
The Minimal Autoinhibited Unit of the Guanine Nucleotide Exchange Factor Intersectin
TLDR
It is proposed that the actual autoinhibited structure contains the related intramolecular SH3(E)-DH interaction, which may block or distort the GTPase binding region of the DH domain.
Phosphorylation of IQGAP1 Modulates Its Binding to Cdc42, Revealing a New Type of Rho-GTPase Regulator*
TLDR
It is proposed that, depending on its phosphorylation state, IQGAP1 might serve as an effector or sequester nucleotide-free Cdc42 to prevent signaling and uncovers a novel type of Rho-GTPase regulator.
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References

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TLDR
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TLDR
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TLDR
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TLDR
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