Endocrine Response Phenotypes Are Altered by Charcoal-Stripped Serum Variability.

@article{Sikora2016EndocrineRP,
  title={Endocrine Response Phenotypes Are Altered by Charcoal-Stripped Serum Variability.},
  author={Matthew J Sikora and Michael D Johnson and Adrian V Lee and Steffi Oesterreich},
  journal={Endocrinology},
  year={2016},
  volume={157 10},
  pages={
          3760-3766
        }
}
Charcoal-stripped bovine serum (CSS) is a critical reagent in the study of steroid hormones. However, CSS has high lot-to-lot variability, including residual growth factor and steroid hormone content. Assessing and reporting this variability is challenging but may affect experimental outcomes and data reproducibility. We hypothesized that CSS lot variability would affect endocrine response phenotypes in breast cancer cells, and we tested the effects of five individual CSS lots on endocrine… CONTINUE READING
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