Endo-exo synergism in cellulose hydrolysis revisited.

  title={Endo-exo synergism in cellulose hydrolysis revisited.},
  author={J{\"u}rgen Jalak and Mihhail Kura{\vs}in and Hele Teugjas and Priit V{\"a}ljam{\"a}e},
  journal={The Journal of biological chemistry},
  volume={287 34},
Synergistic cooperation of different enzymes is a prerequisite for efficient degradation of cellulose. The conventional mechanistic interpretation of the synergism between randomly acting endoglucanases (EGs) and chain end-specific processive cellobiohydrolases (CBHs) is that EG-generated new chain ends on cellulose surface serve as starting points for CBHs. Here we studied the hydrolysis of bacterial cellulose (BC) by CBH TrCel7A and EG TrCel5A from Trichoderma reesei under both single… CONTINUE READING

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