End-targeting proteomics of isolated chromatin segments of a mammalian ribosomal RNA gene promoter

@inproceedings{Ide2015EndtargetingPO,
  title={End-targeting proteomics of isolated chromatin segments of a mammalian ribosomal RNA gene promoter},
  author={Satoru Ide and J{\'e}r{\^o}me D{\'e}jardin},
  booktitle={Nature communications},
  year={2015}
}
The unbiased identification of proteins associated with specific loci is crucial for understanding chromatin-based processes. The proteomics of isolated chromatin fragment (PICh) method has previously been developed to purify telomeres and identify associated proteins. This approach is based on the affinity capture of endogenous chromatin segments by hybridization with oligonucleotide containing locked nucleic acids. However, PICh is only efficient with highly abundant genomic targets, limiting… CONTINUE READING
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