Encephalitozoon cuniculi: quantitation of parasites and evaluation of viability.

@article{Peterson1988EncephalitozoonCQ,
  title={Encephalitozoon cuniculi: quantitation of parasites and evaluation of viability.},
  author={Norman G. Peterson and J A Liu and John A. Shadduck},
  journal={The Journal of protozoology},
  year={1988},
  volume={35 3},
  pages={430-4}
}
Two methods (manual and automated) for quantitation of viable versus dead Encephalitozoon cuniculi are reported. The manual method uses ethidium bromide and acridine orange to stain dead and viable organisms, respectively. The stained organisms are visually differentiated with the aid of a fluorescence microscope. The automated method uses propidium iodide to stain dead parasites, which are differentiated from viable unstained parasites with the aid of a flow cytometer. An automated cell… CONTINUE READING

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