Encephalitogenicity of myelin-associated oligodendrocytic basic protein and 2',3'-cyclic nucleotide 3'-phosphodiesterase for BALB/c and SJL mice.

@article{Mtt1998EncephalitogenicityOM,
  title={Encephalitogenicity of myelin-associated oligodendrocytic basic protein and 2',3'-cyclic nucleotide 3'-phosphodiesterase for BALB/c and SJL mice.},
  author={Jorma M{\"a}{\"a}tt{\"a} and M S K{\"a}ldman and Saburo Sakoda and Aimo A. Salmi and A. E. Hinkkanen},
  journal={Immunology},
  year={1998},
  volume={95 3},
  pages={
          383-8
        }
}
In search of new encephalitogenic myelin antigens, the 2',3'-cyclic nucleotide 3'-phosphodiesterase (CNP) and 19 000 MW isoform of myelin-associated oligodendrocytic basic protein (MOBP) were obtained as recombinant proteins by the baculovirus expression system in Spodoptera frugiperda cells and purified to homogeneity by immobilized metal chelate affinity chromatography (IMAC). The purified MOBP was soluble in water and showed retarded migration on sodium dodecyl sulphate-polyacrylamide gel… CONTINUE READING
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