Encapsulation of Enzymes in Liposomes: High Encapsulation Efficiency and Control of Substrate Permeability

  title={Encapsulation of Enzymes in Liposomes: High Encapsulation Efficiency and Control of Substrate Permeability},
  author={Barnab{\'e} Chaize and Jacques-Philippe Colletier and Mathias Winterhalter and D. Fournier},
  journal={Artificial Cells, Blood Substitutes, and Biotechnology},
  pages={67 - 75}
Enzyme encapsulation into liposomes is a promising technique to stabilize and prevent them from denaturation and proteolysis. We demonstrate this using acetylcholinesterase which is the main target for pesticides. In order to achieve a reasonable encapsulation yield, we analyzed the parameters involved in each step of various encapsulation procedures. The only encapsulation method which did not denature the protein was the lipid film hydration technique, however the encapsulation efficiency was… 

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