Enantioselective capillary electrophoresis-mass spectrometry of amino acids in cerebrospinal fluid using a chiral derivatizing agent and volatile surfactant.

@article{Prior2016EnantioselectiveCE,
  title={Enantioselective capillary electrophoresis-mass spectrometry of amino acids in cerebrospinal fluid using a chiral derivatizing agent and volatile surfactant.},
  author={Amir Prior and R C Moldovan and Jacques Crommen and A C Servais and Marianne Fillet and Gerhardus J Ad de Jong and Govert W Somsen},
  journal={Analytica chimica acta},
  year={2016},
  volume={940},
  pages={150-8}
}
The sensitivity of coupled enantioselective capillary electrophoresis-mass spectrometry (CE-MS) of amino acids (AAs) is often hampered by the chiral selectors in the background electrolyte (BGE). A new method is presented in which the use of a chiral selector is circumvented by employing (+)-1-(9-fluorenyl)ethyl chloroformate (FLEC) as chiral AA derivatizing agent and ammonium perfluorooctanoate (APFO) as a volatile pseudostationary phase for separation of the formed diastereomers. Efficient AA… CONTINUE READING