Enantiomeric separation and sensitive determination of D,L-amino acids derivatized with fluorogenic benzofurazan reagents on Pirkle type stationary phases.

@article{Fukushima1995EnantiomericSA,
  title={Enantiomeric separation and sensitive determination of D,L-amino acids derivatized with fluorogenic benzofurazan reagents on Pirkle type stationary phases.},
  author={Takeshi Fukushima and Masaru Kato and Tomofumi Santa and Kazuhiro Imai},
  journal={Biomedical chromatography : BMC},
  year={1995},
  volume={9 1},
  pages={10-7}
}
The enantiomeric separations of D,L-amino acids derivatized with fluorogenic reagents, 4-fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-F), 4-(N,N-dimethylaminosulphonyl)-7-fluoro-2,1,3-benzoxadiazole (DBD-F) and 4-aminosulphonyl-7-fluoro-2,1,3-benzoxadiazole (ABD-F) by high-performance liquid chromatography (HPLC) on various Pirkle type chiral stationary phases (CSPs, Sumichiral OA series) with citric acid in methanol as a mobile phase were studied. Since the least retention and no separation was… CONTINUE READING