Enantiomeric determination of tramadol and O-desmethyltramadol in human plasma by fast liquid chromatographic technique coupled with mass spectrometric detection.

@article{Chytil2010EnantiomericDO,
  title={Enantiomeric determination of tramadol and O-desmethyltramadol in human plasma by fast liquid chromatographic technique coupled with mass spectrometric detection.},
  author={Luk{\'a}{\vs} Chytil and Olga Matou{\vs}kov{\'a} and Olga {\vC}ern{\'a} and Pavla Pokorn{\'a} and V{\'a}clav Vobruba and F Perl{\'i}k and O Slanař},
  journal={Journal of chromatography. B, Analytical technologies in the biomedical and life sciences},
  year={2010},
  volume={878 3-4},
  pages={481-6}
}
A rapid and sensitive method using liquid chromatography-tandem mass spectrometry (LC-MS/MS) for enantiomeric determination of tramadol and its primary phase metabolite O-desmethyltramadol in human plasma has been developed. Tramadol hydrochloride-(13)C, d(3), was used as an isotopic labeled internal standard for quantification. The method involves a simple solid phase extraction. The analytes and internal standard were separated on Lux Cellulose-2 packed with cellulose tris(3-chloro-4… CONTINUE READING

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