En bloc immunohistochemistry reveals extensive distribution of histidine decarboxylase-immunoreactive neurons on the ventral surface of the rat hypothalamus.

@article{Reiner1987EnBI,
  title={En bloc immunohistochemistry reveals extensive distribution of histidine decarboxylase-immunoreactive neurons on the ventral surface of the rat hypothalamus.},
  author={P. Reiner and Kazue Semba and Takeshi Watanabe and Hiroshi Wada},
  journal={Neuroscience letters},
  year={1987},
  volume={77 2},
  pages={137-42}
}
En bloc immunohistochemistry was used to examine the distribution of L-histidine decarboxylase (HDC)-immunoreactive neurons on the ventral surface of the rat hypothalamus. Following standard paraformaldehyde fixation, the ventral hypothalamus containing the tuber cinereum was carefully dissected free and incubated en bloc with antisera to HDC followed by… CONTINUE READING