Employing phage display to study the mode of action of Bacillus thuringiensis Cry toxins.

@article{Fernndez2008EmployingPD,
  title={Employing phage display to study the mode of action of Bacillus thuringiensis Cry toxins.},
  author={Luisa Elena Fern{\'a}ndez and Isabel C G{\'o}mez and Sabino Pacheco and Iv{\'a}n Arenas and Sarjeet S Gilla and Alejandra Bravo and Mario Sober{\'o}n},
  journal={Peptides},
  year={2008},
  volume={29 2},
  pages={
          324-9
        }
}
Phage display is an in vitro method for selecting polypeptides with desired properties from a large collection of variants. The insecticidal Cry toxins produced by Bacillus thuringiensis are highly specific to different insects. Various proteins such as cadherin, aminopeptidase-N (APN) and alkaline phosphatase (ALP) have been characterized as potential Cry-receptors. We used phage display to characterize the Cry toxin-receptor interaction(s). By employing phage-libraries that display single… CONTINUE READING

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