Empirical assessment of RAD sequencing for interspecific phylogeny.

@article{Cruaud2014EmpiricalAO,
  title={Empirical assessment of RAD sequencing for interspecific phylogeny.},
  author={A. Cruaud and M. Gautier and M. Galan and J. Foucaud and L. Saun{\'e} and G. Genson and E. Dubois and S. Nidelet and T. Deuve and J. Rasplus},
  journal={Molecular biology and evolution},
  year={2014},
  volume={31 5},
  pages={
          1272-4
        }
}
Next-generation sequencing opened up new possibilities in phylogenetics; however, choosing an appropriate method of sample preparation remains challenging. Here, we demonstrate that restriction-site-associated DNA sequencing (RAD-seq) generates useful data for phylogenomics. Analysis of our RAD library using current bioinformatic and phylogenetic tools produced 400× more sites than our Sanger approach (2,262,825 nt/species), fully resolving relationships between 18 species of ground beetles… Expand
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